Abul Bashar Ripon Khalipha1, 2*
1 Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University,
Gopalganj 8100,
2 Evergreen Scientific Research Centre, Gopalganj-8100, Bangladesh
Abstract
Local tribes in East Libya frequently make use of a medicinal plant known as Arbutus pavarii Pamp for the treatment of a wide variety of conditions, including gastritis, renal infections, cancer, and kidney ailments. The purpose of this research was to explore the antibacterial activity of the plant’s flowers and roots tracts against methicillin resistant Staphylococcus aureus (MRSA), as well as the metabolite profiles of the bioactive fractions. The disc diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were used to determine the antibacterial activity, while the time kill test was used to evaluate the microbial decrease caused by the bioactive fraction. An ultrahigh-performance liquid chromatography mass spectrometry (UHPLC-ESI-MS/MS) study was performed on the bioactive fraction to putatively identify the chemical ingredients that were present within it. On the several MRSA strains that were examined, each of the extracts and fractions shown varying degrees of antibiotic activity. The crude flowers methanolic extract displayed the greatest amount of overall antibacterial activity, which was calculated to be 4007.6 mL/g. In contrast, the ethyl acetate fraction of the flowers exhibited moderate to considerable antibacterial activity against MRSA at concentrations as low as 0.08–1.25 mg/mL of the minimal inhibitory concentration. Following metabolite profiling of this fraction with UHPLC-ESI-MS/MS, a total of 28 compounds, including phenolic acids, flavan-3-ols, and flavonols, were found to have a strong
possibility of being identified. According to the findings of this study, the ethyl acetate fraction of Arbutus pavarii flowers held the potential for antibacterial activity against MRSA. As a result, this natural
antibacterial agent can be further investigated for possible applications in the pharmaceutical industry