Rajat Bagchi1, Abul Bashar Ripon Khalipha1,2*, Sadia Afrin1 , Milon Mondal1, Md. Solayman Hossain11Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science & Technology University, Gopalganj,
Bangladesh
2Evergreen Scientific Research Centre, Gopalganj-8100, Bangladesh

Abstract
Litsea glutinosa, belonging to the family lauraceae, is traditionally used in the
treatment of diarrhea, dysentery, joint ache, skin infection, respiratory sickness,
etc. The prevailing study was designed to investigate the bioactive chemical
compounds present in the methanolic extract of L. glutinosa bark through GC-MS
analysis and examine castor oil-triggered anti-diarrheal activity in swiss albino
mice. A total of 21 phytoconstituents were recognized via GC-MS evaluation
from the bark extract. The major components identified inclusive of 1,4
Anhydro-d-mannitol, 13-Docosenamide, 2,4-di-tert-butyl phenol, 9,12
Octadecadienoic acid, 9-Octadecenoic acid, 1H-Indole-2-carboxylic acid,
hexadecanoic acid and many others. In the antidiarrheal activity test, the bark
extract 250 mg/kg and 500 mg/kg extended the onset of diarrhea and showed
significant % inhibition of defecation, 37.93% (P<0.01) and 63.79% (P<0.001)
respectively in a dose dependent manner evaluate to control. The standard drug,
loperamide, prominently reduced the occurrence of diarrhea and exhibited
effective % inhibition of defecation (65.52%) in the experimental animals. The
outcomes confirmed that the methanolic extract of this plant contains vital
bioactive components and possesses substantial anti-diarrheal activity, which
rationalised the conventional use of the plant.
Keywords: Litsea glutinosa, GC-MS, 13-Docosenamide, anti-diarrheal.